Seroprevalence of contagious bovine pleuropneumonia (CBPP) in cattle from Karamoja region, North-eastern Uganda.

BACKGROUND: Contagious bovine pleuropneumonia [CBPP] is a transboundary animal disease of cattle caused by Mycoplasma mycoides subsp. mycoides [Mmm]. CBPP causes severe economic losses to livestock producers in sub-Saharan Africa mainly due to high mortality, morbidity, reduction in productivity as well as livestock trade restrictions. This study aimed at determining seroprevalence of Mmm in cattle from Karamoja region, north-eastern Uganda; data that are required to design and implement risk based CBPP control program. METHODS: We randomly collected blood samples from 2,300 cattle spread across Karamoja region. Serum was extracted and screened for antibodies against Mycoplasma mycoides subsp. mycoides [Mmm] using the competitive enzyme linked immunosorbent assay [cELISA]. RESULTS: A quarter [25.4%; 95% CI: 23.7-27.3] of the screened cattle [n = 2,300] were sero-positive for Mmm. Amudat and Kaabong districts recorded the lowest [12.3%] and highest [30.7%] Mmm seroprevalence respectively. Increasing age, overnight stay in cattle kraals and location [certain districts, villages, herds and sub counties] of the cattle herds, the factors that promote animal commingling, were the most significant risk factors of seroconversion with Mmm. CONCLUSION: Results from this study indicated a higher seroprevalence of Mmm in Karamoja region cattle herds. This could be due to the increased frequency of CBPP outbreaks in recent years. To be effective, CBPP vaccination programs should target high risk herds along the international borders and other hotspot areas [e.g., parishes or sub counties] where cattle commingling is high.

Mycoplasma mycoides subspecies capri, an uncommon mastitis and respiratory pathogen isolated in a German flock of goats.

Mycoplasma mycoides ssp. capri (Mmc) is one of the etiological microorganisms of contagious agalactia, which is among the diseases causing the highest economical losses in small ruminants. We report a disease outbreak in a German flock that led to significant suffering of goats characterized by mastitis, arthritis, pleuropneumonia and sudden deaths. Mmc was persistently isolated from many animals both from milk, and from a number of different swab and tissue samples. A number of closely related Mycoplasma spp. have to be taken into consideration to rule out important animal epizootics listed by European Animal Health Law and the World Organisation for Animal Health (WOAH). Some goats developed cross-reacting antibodies against Mycoplasma mycoides ssp. mycoides. Although Mmc is believed to be an uncommon microorganism in Germany, this study highlights that veterinarians should consider this pathogen in their work during herd health monitoring in Central Europe. Although eradication was not fully achieved, autogenous vaccination significantly seemed to improve animal health and welfare.

Sero-prevalence of contagious bovine pleuropneumonia in dryland of Borana, southern Oromia, Ethiopia.

Ethiopia is one of the largest African countries where livestock farming represent a relevant resource for the economy and the livelihood of the population. Contagious bovine pleuropneumonia (CBPP) is among the transboundaries animal disease that is hindering cattle farming in Ethiopia. Due to the limited resources of veterinary services, disease control and surveillance is discontinuous and occasional field investigations of target areas contribute to depict disease spreading in the country. The study was conducted to determine the seroprevalence, at herd and animal level, and identify the risk factors involved in CBPP diffusion and persistence in the Borana pastoral zone. A total of 498 serum samples were collected from 120 cattle herds and tested using competitive Enzyme-Linked Immunosorbent Assay (c-ELISA). Of 120 herds sampled, 37 (30.83%; (95% CI = 22.73-39.91%) were tested positive to CBPP antibody. Out of 498 sera samples tested 46 (9.24%; 95% CI = 6.84-12.13%) were positive. The highest prevalence was observed in Teltele (12/95; 12.90%; 95% CI = 6.7-21%) followed by Yabello (12/104; 11.54%; 95% CI = 6.1-19.3%) and Arero (10/91; 10.99%; 95% CI = 95% CI = 5.4-19.3%), whereas the lowest prevalence was observed in Gomole (5/101; 6.42%; 95% CI = 1.6-11.2%) and Dubluk (7/109; 4.95%; 95% CI = 2.6-12.8%) districts and statistically not significant (p > 0.05). Results of multivariate logistic regression analysis revealed that, age, herd movement and herd size of the animals had statistically significant effect on sero-positivity to CBPP (p < 0.05). Sex, season and body condition were not significantly (p > 0.05) associated with the occurrence of CBPP. The study confirms that CBPP is persistent in the territory and remain as a major problem that affects health and productivity of cattle. Therefore, awareness creation to the pastoralists in the study area about the effect of CBPP and designing appropriate control methods has a paramount importance to improve the health and productivity of cattle production in the area.

Risk factors associated with contagious caprine pleuropneumonia in goats of Amhara region, Ethiopia.

Contagious caprine pleuropneumonia (CCPP) is a serious contagious disease of goats, sheep and wild ruminants caused by Mycoplasma capricolum subspecies capripneumoniae. The disease is known for its high mortality, morbidity and economic losses. A cross-sectional study using multistage cluster sampling technique was conducted in Amhara region from January 2019 to July 2019 to estimate seroprevalence and identify risk factors of CCPP occurrence in the region. A total of 2080 goats from 61 villages and 12 districts of the region were tested for CCPP serostatus using Competitive Enzyme-Linked Immunosorbent Assay (C-ELISA). A multilevel mixed-effect logistic regression model was used to identify risk factors of CCPP seropositivity at animal and flock-level. The serum sample results revealed an overall animal level seroprevalence of 5.1% (95% CI: 3.8-6.6) and flock-level prevalence of 26.0% (95% CI: 19.7-33.4). At individual animal level, presence of other health problems (OR = 45.9 (95% CI: 25.3-83.4)), age (adult age (OR = 6.2 (95% CI:3.4-11.4)) and old age (OR = 13.1 (95% CI: 6.2-27.8))), and breed type (Afar (OR= 32.3 (95% CI: 2.9-366.1)), Central highland (OR=13.7 (95% CI: 1.3-140.6)), and western highland (OR=16.2 (95% CI: 1.4-185.7))) were identified as risk factors for CCPP seropositivity. In contrast, contact with other flocks (OR = 59.9 (95% CI: 6.1-585.6)), presence of trade route (OR = 3.1 (95% CI: 1.0-9.1)) and presence of sheep (OR = 2.6 (95% CI: 1.2-5.7)) were flock-level risk factors for CCPP seropositivity. Generally, CCPP appears to be common among goats of Amhara region. Goat flocks dominated with older age animals; breeds of Afar, central highland, and western highland; raise with sheep; have contact with other flocks; and kept along trade routes are more at risk for CCPP. Hence, awareness creation to the producers, movement control, and regular prophylactic vaccination should be considered to control CCPP in Amhara region.

Multi-locus sequence analysis reveals great genetic diversity among Mycoplasma capricolum subsp. capripneumoniae strains in Asia.

Multi-Locus Sequence Analysis (MLSA) of Mycoplasma capricolum subsp. capripneumoniae (Mccp) strains from Asia revealed unforeseen diversity and a central position for genotyping groups representing strains from Central/East Asia, suggesting a possible origin of contagious caprine pleuropneumonia in this continent. A better assessment of the emergence, diversity and distribution of Mccp in Asia and Africa calls for renewed efforts to dramatically enlarge the sample of strains. Availability and affordability in the field, added to superior typeability (directly from poor samples) and high stability, discriminatory power and concordance with epidemiological and phylogenetic analyses, make MLSA an excellent tool for such investigations.

Seroepidemiology of selected transboundary animal diseases in goats in Zambia.

Transboundary pathogens of goats present significant constraints to the livelihoods of millions of farmers in countries such as Zambia. Consequently, this study aimed to investigate the seroprevalence of Mycoplasma capricolum subsp. capripneumoniae (Mccp), foot and mouth disease virus (FMDV), Brucella spp., Crimean Congo haemorrhagic fever virus (CCHFV), and Rift Valley fever virus (RVFV) in Zambian goats. Another aim was to identify associations between seroprevalence and different predictor variables, such as trade and border proximity. From September to October 2019, 962 serum samples were collected from goats in seven Zambian districts, four of which have an international border while the remaining three do not. A questionnaire survey was conducted with each household, focusing on trade routines, management strategies and herd disease history. Animal-level seroprevalence adjusted for herd-level clustering was 8.2 % (95 % confidence interval [CI] 7.5-9.0) for Mccp, 12.9% (95% CI 12.0-13.7) for FMDV, 13.0 % (95% CI 12.1-13.9) for Brucella spp., 3.3 % (95% CI 2.8-3.7) for CCHFV, and 0.4 % (95 % CI 0.3-0.7) for RVFV. The association between herd-level seroprevalence and border proximity and trade appeared negligible, with the exception of selling goats at least twice a year which was identified as a potential risk factor for Brucella spp. (OR 4.1, 95 % CI 1.1-16.0, p = 0.040). In addition, a positive association between herd-level seroprevalence of FMDV and a herd size of 21 goats or more (OR 3.3, 95 % CI 1.0-11.1, p = 0.049) was detected. Also, positive associations between animal-level seroprevalence of Brucella spp. and increasing age (OR 7.7, 95 % CI 1.5-40.7, p = 0.016), and CCHFV and keeping pigs in the household (OR 2.7, 95 % CI 1.0-7.1, p = 0.044), were found. For FMDV (OR 3.8, 95 % CI 1.4-10.9, p = 0.011) and Brucella spp. (OR 4.5, 95 % CI 1.2-17.3, p = 0.031) on the other hand, animal-level seroprevalence was significantly higher in households without pigs. To the best of the authors' knowledge, this is the first study to describe the presence of antibodies for CCPP and CCHF in the Zambian goat population. While the association between seroprevalence and trade and border proximity generally appeared negligible, it is recommended that their influence is further evaluated in future studies, preferably through in-depth longitudinal studies incorporating impacts of different biosecurity measures and trade variations, linked to for example seasonality and trade peaks.

Contagious bovine pleuropneumonia: Seroprevalence and its associated risk factors in selected districts of Afar region, Ethiopia.

BACKGROUND: In pastoral and lowland areas of the country particularly in Afar region, studies suggested higher prevalence of contagious bovine pleuropneumonia (CBPP) than mid and highland agro-ecologies. Though CBPP is a prime constraint to cattle productivity in the region, research outputs pertaining to CBPP are unavailable compared to highland areas. Thus, the objectives of the current study were to determine seroprevalence of CBPP and assess risk factors in selected districts of Afar region. METHODS: A cross-sectional study was conducted on cattle aged 6 months and above from February 2018 to January 2019 in selected districts of the region. A total of 420 blood samples were collected and sera were separated for further serologic analysis. Using competitive enzyme linked immunosorbent assay (c-ELISA), antibodies against Mycoplasma mycoides subspecies mycoides small colony (MmmSc) were detected at National Veterinary Institute, Ethiopia. Data were analysed using Stata version 14.0. RESULT: Of 420 samples tested by c-ELISA, 158 samples were found to be positive for CBPP providing an overall seroprevalence of 37.6%. Among the three risk factors considered (age, sex and district) assessed, only two (age and district) were found to be associated significantly with the disease (p < 0.05) at 95% CI and p-value less than 5% applying logistic regression. CONCLUSION: The study has revealed a higher prevalence of CBPP over the study areas urging a coordinated act to be set in place.

Contagious caprine pleuropneumonia (CCPP): A systematic review and meta-analysis of the prevalence in sheep and goats.

Contagious caprine pleuropneumonia (CCPP) is a highly contagious respiratory disease of small ruminants that is caused by the bacterium Mycoplasma capricolum subsp. capripneumoniae. Sheep and goats are two of the species of small ruminants most at risk of CCPP. Outbreaks of CCPP cause significant economic and trade disturbances in several parts of the world. However, the extent and magnitude of CCPP in a particular geographical region is still not well known due to lack of comprehensive data on its occurrence. The present study aimed to investigate the prevalence of CCPP in sheep and goats raised in different geographical regions as well as the factors contributing to the spread of the disease. Searches were done in five online repositories: PubMed, Web of Science, Scopus, CAB Direct and Google Scholar using pre-selected key terms. Data were retrieved from the 41 articles that met the study's inclusion criteria. The pooled prevalences were determined using a random effect meta-analysis model. Prevalence of CCPP was 23.19% (95% CI: 11.90-34.47%) in sheep and 24.91% (95% CI: 20.99-28.84%) in goats. Overall, the regional level pooled prevalence estimates ranged from 8.0% (95% CI: 6.91-9.09%) to 28.70% (22.02-35.38%), depending on species and world region. Substantial heterogeneity (I2  > 75%) was observed in most pooled prevalence estimates. The results indicate high prevalences of CCPP in sheep and goats across the regions, particularly in Africa and Asia, and highlights the need to institute appropriate control measures. Active surveillance and research on risk factors are recommended.

[Present Situation of Contagious Bovine Pleuropneumonia in Central African Republic]. / Situation Actuelle de la Péripneumonie Contagieuse Bovine en République Centrafricaine.

Aims: The aim of this study was to analyse the current situation of Contagious Bovine Pleuropneumonia (CBPP) in the Central African Republic (CAR) by seroprevalence analysis, as well as isolation and characterization of strains of the etiologic agent, Mycoplasma mycoides mycoides (Mmm), circulating in livestock breeding regions. Material and methods: The strains obtained were subjected to whole genome sequencing by Illumina technology and genotyped using the eMLST technique based on 62 genes of the Mmm core genome. Their sensitivity to tetracycline was assessed by determination of the minimum inhibitory concentration (MIC) on agar. A seroprevalence analysis by competitive ELISA was conducted in livestock breeding regions (West, Centre and East CAR), including both zebu and taurine cattle breeds, and both males and females. Results: The three strains isolated in the three regions of the CAR shared exactly the same genomic sequence. Phylogenetic analysis showed that they were closely related to a strain isolated in the CAR in 1991, also sequenced in this study, and clustered with Mmm strains originating from East and Central Africa. The recent isolates presented increased MIC values, though they were still sensitive to tetracycline. The global CBPP prevalence in the CAR was estimated at 12.5% with no significant differences observed between cattle breeding regions, nor between males and females. However, a significantly higher prevalence was observed in zebu compared to taurine cattle, most likely in relation to their herding system based on cattle transhumance and nomadic pastoralism. Conclusion: CBPP is enzootic in the CAR in spite of control campaigns based on use of the live T1 vaccines, which have shown little efficacy due to poor implementation in the field. New strategies combining controlled use of antibiotics and inactivated vaccines, with increased thermostability, should be well received by livestock keepers and allow a better control of CBPP in the region. The fact that the recent Mmm isolates are still resistant to tetracycles is encouraging.

Seroprevalence and determinants of contagious bovine pleuropneumonia in cattle in Burkina Faso.

Contagious bovine pleuropneumonia (CBPP) is a bacterial disease caused by Mycoplasma mycoides subsp. Mycoides. This disease affects ruminants mainly cattle with respiratory disorders as predominant symptoms. In Burkina Faso, this condition has been considered as enzootic since several years but data on its seroprevalence remains scares. This study aimed to establish the serological prevalence and determinants of CBPP in Burkina Faso in 2017. For this purpose, 3969 serum samples have been collected following a stratified sampling plan based on vaccination coverage in 12 regions, 84 communes, and 210 villages and analyzed using c-ELISA test. Individual seroprevalence was 16.91% (95% CI: 15.74-18.07%), while 84.5% (95% CI: 60.46-80.02%) of communes, chosen as epidemiological units were found positive. The individual prevalence was found to be associated with agro-ecological area (p < 0.05) and a prevalence of 18.70% (95% CI: 16.74-20.66%) was noted in Sahelian areas, while 15.79% (95% CI: 14.34-17.23%) was found in Soudanian areas. The prevalence was also significantly associated with vaccination coverage (p < 0.05) with a prevalence of 13.92% (95% CI: 11.66-16.18%), 19.21% (95% CI: 16.66-20.75%) and 11.61%(95% CI: 9.00-14.23%) for high, moderate, and low vaccination coverage respectively. The individual prevalence was respectively 16.97 (95% CI: 15.56-18.39%) and 17.13% (95% CI: 15.93-18.33%) for female and animals more than 2 years old. According to regions, the highest seroprevalence was found in Plateau Central region (38.18%, 95% CI: 29.1-47.26%), while the lowest was found in Centre-Est Region (7%, 95% CI: 4.5-9.5%). These prevalence data will allow us to adapt the ongoing strategy to control CBPP in Burkina Faso.

The first identification of contagious caprine pleuropneumonia (CCPP) in sheep and goats in Egypt: molecular and pathological characterization.

Contagious caprine pleuropneumonia (CCPP) is one of the most fatal and contagious diseases of goats. To date, the occurrence of CCPP in Egypt has not been reported. During the period from 2017 to 2018, 200 goats and 400 sheep from Matrouh Governorate (Al Alamein and El Hammam cities) were suspected to have CCPP; animals were examined to confirm the presence of CCPP infection as well as the epidemiological status, clinical features, and molecular and histopathologic characteristics of lung tissues. Additionally, a treatment trial was performed to assess the efficacy of anti-mycoplasma therapy in the treatment of clinical cases of this disease. The occurrence of CCPP was 32.5% and 5% in goats and sheep, respectively, while case fatality was 30% and 8% in goats and sheep, respectively. The clinical forms of CCPP in both sheep and goats varied from per-acute to acute or chronic cases. Histopathological analysis of lung tissues from dead cases (either sheep or goats) revealed different stages of broncho- and pleuropneumonia ranging from per-acute to acute or chronic stages. Lung tissues showed severe congestion of interalveolar capillaries, flooding of alveoli and bronchi with a fibrinous exudate, a high degree of pleural thickening, and multifocal areas of necrosis that were sometimes sequestered in the fibrous capsule. Isolation of Mycoplasma capricolum subspecies capripneumoniae (Mccp) was confirmed in all dead cases by agar and broth culture methods and polymerase chain reaction. The treatment trial revealed that the marbofloxacin and spiramycin groups had a higher cure rate (70%) than the oxytetracycline group (40%) and a lower fatality rate (30%) than the oxytetracycline group (60%). Conclusively, infection with CCPP in goats and sheep is considered to be novel for Mccp in Egypt, where this species is considered to be the main pathogen in goats, not in sheep. Additionally, it could be concluded that treatment may be effective only if given early. Further comprehensive surveys are required to investigate the risk of CCPP in goats and sheep in all Egyptian governorates.

Economic impact of contagious caprine pleuropneumonia and cost-benefit analysis of the vaccination programmes based on a one-year continuous monitoring of flocks in the arid and semi-arid lands of Kenya.

In Kenya and East Africa, contagious caprine pleuropneumonia (CCPP) is one of the most prevalent infectious diseases affecting small ruminants in pastoral areas with adverse consequences on livelihoods. This is so despite the implementation of bi-annual vaccination campaigns. Unfortunately, the impact of the disease and the cost-effectiveness of its prevention and control in a pastoral context have been difficult to assess due to a lack of reliable data. The dynamic of flock population, high illiteracy and limited outreach are the main challenges for proper data collection. Nevertheless, such analysis is important to justify the implementation of national vaccination campaign for livestock disease control and to contribute to pastoral households' economy support programme. A continuous flock monitoring was performed for a year in Turkana County to collect data on flock dynamics and the different causes of mortalities. A stochastic model was developed to evaluate the annual economic losses due to CCPP in a standard flock of 100 heads and evaluate the cost-benefit ratio of the vaccination programmes based on different scenarios of 95%, 50% and 20% vaccination effectiveness. The annual economic losses due to CCPP for a standard flock of 100 heads were estimated at Euros 1,712.66 in average. The benefits-costs ratio of the vaccination supports the current bi-annual vaccination campaigns, even with a vaccine effectiveness limited to 20% (average benefits-costs ratio of 5.715 with SD of 3.914). This justifies the campaigns as part of a food security or livelihood support programme. However, from an overall health perspective and for long-term effects on livestock asset protection and disease control, a higher vaccination effectiveness is required.

Risk factors associated with Mycoplasma capricolum subspecies capripneumoniae and morbillivirus infection in small ruminants in Tanzania.

Mortality of domestic small ruminants caused by contagious caprine pleuropneumonia (CCPP) and Peste des petits ruminants (PPR) is frequently reported in Tanzania. A cross-sectional survey was conducted between June, 2016 and July, 2017 to identify risk factors for small ruminants exposure to Mycoplasma capricolum subsp. capripneumoniae (M. capripneumoniae), the causative agent of CCPP, and small ruminant morbillivirus (SRMV), the causative agent of PPR. Antibody detection was done using competitive enzyme-linked immunosorbent assays (cELISA); similarly, a semi-structured questionnaire was administered in flocks where serum samples were collected. Individual seropositivity for M. capripneumoniae was 6.5% (n = 676) and 4.2% (n = 285) in goats and sheep respectively, whereas SRMV was 28.6% in goats (n = 676) and 31.9% in sheep (n = 285). Multivariable analysis indicated that mixing of flocks was a risk factor for exposure to M. capripneumoniae (χ2 = 3.9, df = 1, p = 0.05) and SRMV (χ2 = 6.3, df = 1, p = 0.01) in goats. Age was a protective factor for SRMV seropositivity in both goats (χ2 = 7.4, df = 1, p = 0.006) and sheep (χ2 = 10.2, df = 1, p = 0.006). SRMV seropositivity in goats was also influenced by grazing in contact with wild animals (χ2 = 5.9, df = 1, p = 0.02) and taking animals to the animal markets (χ2 = 8.2, df = 1, p = 0.004). M. capripneumoniae and SRMV are influenced by several risk factors and their control needs concerted efforts between stakeholders, which may include community involvement in mandatory vaccination and animals' movement control.

An outbreak of Mycoplasma mycoides subspecies capri arthritis in young goats: a case study.

Mycoplasmosis is a well-known cause of morbidity and mortality in small ruminants. Previously recognized outbreaks have involved arthritis, and pneumonia or pleuropneumonia. Modern bacteriology procedures rely less on isolation techniques that require special media for mollicutes given that these species are notoriously difficult to isolate, and rely more on PCR tests. We report an outbreak of arthritis, pleuropneumonia, and mild meningitis affecting dairy goat kids, spanning a period of 3 y, which had unusual epidemiologic characteristics related to husbandry practices. Lesions were characterized by polyarthritis of the appendicular joints, with copious joint fluid and extension of arthritic exudate beyond the joint itself. The cause remained unknown until serendipitous isolation of a mycoplasma on blood agar. Mycoplasmosis was not detected from synovial samples by a general mycoplasma PCR, despite multiple attempts. Isolated colonies were also negative by this general PCR assay. The isolate was identified as Mycoplasma mycoides subspecies capri, using universal 16S primers and amplicon sequencing. Testing of additional isolates from other diseased goats in the herd confirmed that this was the cause of illness. A failure to recognize the distinct nature of organisms of the M. mycoides group of mycoplasmas meant that a PCR test that cannot detect this group of organisms was utilized at first, and the etiology of the illness was overlooked for a period of time. Veterinary pathologists and microbiologists must be aware of the limitations of some PCR assays when confronted with joint disease and pleuropneumonia in small ruminants.

Information sharing and willingness-to-pay for CBPP vaccine in rural Kenya.

The study estimates cattle owners' willingness-to-pay (WTP) for Contagious Bovine Pleuropneumonia (CBPP) vaccine in Samburu county, Kenya. Of particular policy relevance, the study presents findings on WTP for i) improved access to vaccines and ii) timely access to disease-risk information. The mean price for a CBPP vaccine was estimated at KES 66 (USD 0.64). This price relates to a CBPP vaccine that requires a 1.8 h commute, cattle owners' receipt of timely information that the CBPP disease risk is low-moderate and the vaccine lowers the risk of either tail-drop or post-vaccine abortion. The conditional WTP for mean travel duration and high-risk information are similar at KES 53.9 and KES 51.5. The marginal effect on demand for a 1 h additional travel duration and provision of CBPP disease risk information was estimated as a 1.5 per cent reduction and 2.3 increase. The results of this study indicate that cattle owners value greater levels of knowledge concerning the changing risk profile of CBPP in their community and improved access to CBPP vaccination services. Enhanced engagement with cattle owners concerning CBPP would likely result in a greater utilisation of available CBPP vaccines, conditional on the perceived CBPP disease risk.

Epidemiological investigations of contagious caprine pleuropneumonia in selected districts of Borana zone, Southern Oromia, Ethiopia.

From November 2016 to April 2017, a cross-sectional study to determine the sero-prevalence of contagious caprine pleuropneumonia (CCPP) and to investigate its epidemiology was conducted in selected districts of Borana zone in Ethiopia. In addition, the study aimed at identifying Mccp antigens using species specific primer of PCR. A multistage random sampling was implemented to select districts, pastoral associations (villages), and households. A total of 890 serum samples of small ruminants that had not been vaccinated (goats n = 789 and sheep n = 101) were collected and screened for the presence of antibodies against Mycoplasma capricolum subspecies capripneumoniae using a competitive enzyme-linked immunosorbent assay. Lung tissues and pleural fluid samples were collected from 3 sero-positive and clinically suspected goats for isolation of Mycoplasma capricolum subspecies capripneumoniae. Serology showed that overall 31.2% (246/789) of goats and 12.9% (13/101) of sheep were positive with statistically significant differences between districts (p = 0.001). Multivariable logistic regression analysis revealed that goats from Moyale and Yabello districts had higher odds of being positive than goats from Elwoya district with odd ratios of 2.05 and 1.61, respectively. Age of goats was also significantly associated with sero-positivity (OR = 1.47; CI 95% 1.2-1.8). Mycoplasma capricolum subspecies capripneumoniae was identified in 6 (75%) of the tissue samples using species-specific primer of PCR. Besides improving the understanding of the epidemiology of CCPP in the selected districts and demonstrating its wide distribution, the study highly also provides evidence of the possible role of sheep in the maintenance of the disease.

Unexpected field observations and transmission dynamics of contagious caprine pleuropneumonia in a sand gazelle herd.

Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae, has long been considered a goat-specific disease. Since 2007 there has been growing evidence that this disease can affect wild ungulates either kept in captivity or in the wild. In 2013, a large collection of sand gazelles (Gazella marica) held in the United Arab Emirates suffered heavy losses due to a CCPP epizootic confirmed by PCR and isolation. Animals displayed typical lesions, with unilateral pneumonia and profuse pleurisy. An initial antibiotic treatment consisting of tylosin administered in drinking water did not improve the animals' condition and vaccination failed to stop the spread to contiguous pens. A treatment with tetracycline mixed in feed pellets finally succeeded to stop the evolution of the disease. A subsequent vaccine trial, performed on naïve animals, showed that only a reference CCPP vaccine produced according to OIE standards induced a sero-conversion by CCPP competition ELISA, while the commercially available vaccines did not. A SEIRD compartment transmission model was developed to better understand the dynamics of the disease. The parameters were initially set as per expert opinion and then adjusted to fit the observed mortality data. The basic reproductive number R0 was estimated to be between 2.3-2.7, while the final mortality rate reached up to 70% in some pens. Transmission of infectious droplets from an external source, through a distance of at least the 50 m separating the pens from the perimeter fence, remains the most plausible explanation for the contamination of this stock of gazelles.

Detection and Isolation of Mycoplasma capricolum Subspecies Capricolum from East Azerbaijan Sheep Flocks.

Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefaciens (Mp) produce a clinically similar disease, more often in goats. The aim of the present study was to detect Mcc in sheep flocks in East Azerbaijan Province of Iran. Milk, ear canal, and eye swab samples were collected from 49 sheep flocks with clinical signs of CA or a history of a disease. All the samples were examined using both culture and molecular methods. In the molecular method,positive samples for the Mycoplasma genus were tested for M. mycoides cluster and Mcc. From 272 samples, 67, 87, and 62 samples were shown to be positive using the culture method, polymerase chain reaction (PCR) method, and both culture and PCR methods, respectively. Mcc was detected in all the four M. mycoides cluster positive samples, including milk, ear canal, and eye swab samples. This is the first report of Mcc detection from East Azerbaijan. Our results showed that eye, milk, and ear canal samples could be suitable sources for Mcc detection in sheep flocks.

Risk factors associated with contagious caprine pleuro-pneumonia in goats in pastoral areas in the Rift Valley region of Kenya.

A cross-sectional study to determine risk factors associated with sero-prevalence of contagious caprine pleuro-pneumonia (CCPP) in goats was carried out between the months of March, 2014 and March, 2015 in Pokot East, Turkana West and Kajiado Central Sub-counties. A semi-structured questionnaire focusing on risk factors for CCPP was completed for each flock whose serum samples were collected. A logistic regression model was developed to assess the association between the risk factors and CCPP sero-positivity. Of the 54 flocks, 49 (90.7%) presented at least one sero-positive animal. Two hundred and four of the 432 goats tested sero-positive at monoclonal antibody based competitive Enzyme-linked immuno-sorbent assay (c-ELISA), hence a sero-prevalence of 47.2% (95% CI=42.5- 51.9). Previous exposure of flocks to CCPP (p<0.001, OR=52.8; CI=6.45, 432), distant sources of veterinary drugs (p<0.001, OR=6.17; CI=3.41, 11.1), movement of goats to dry season feeding areas (p<0.001, OR=4.31; CI=2.39, 7.75) and markets as a source of new introductions to the flock (p=0.033, OR=1.86; CI=1.05, 3.27) were identified as risk factors significantly associated with CCPP sero-prevalence. The findings provide further evidence supporting the high prevalence and endemic state of the disease in pastoral flocks and hence there is need for adequate measures to be put in place to control the disease effectively.

Contagious bovine pleuropneumonia: Seroprevalence and risk factors in Western Oromia, Ethiopia.

Contagious bovine pleuropneumonia (CBPP) is one of the most important threats to cattle health and production in Ethiopia. At the livestock farm of the Bako Agricultural Research Center, an outbreak of respiratory disease of cattle occurred in May 2011, and many animals were affected and died before the disease was diagnosed. Therefore, this study was designed to determine the seroprevalence of CBPP antibodies in selected districts of Western Oromia Region and to assess the potential risk factors for the occurrence of the disease. A crosssectional study was conducted from November 2013 to March 2014 in three selected districts of Western Oromia Region. A total of 386 sera were examined for the presence of specific antibodies against Mycoplasma mycoidesmycoides small colony (MmmSC), using a competitive enzyme-linked immunosorbent assay. The risk factors that were evaluated in this study were geographical location, age, sex, breed and body condition. The overall seroprevalence in this study was 28.5%. The seroprevalence of Mycoplasma mycoidesmycoides small colony antibodies at the district level was 40.3%, 19.0% and 5.7% in Gobbu Sayyo, BakoTibbe and Horro districts, respectively. There was a statistically significant variation ( p < 0.05) in the prevalence of antibodies amongst the districts. However, animal-related risk factors, such as age, sex, breed and body condition, were not significantly associated ( p > 0.05) with the serological status of the animal. This study showed that the overall prevalence of CBPP in Western Oromia Zones was high. This warrants the implementation of appropriate preventive and control measures to minimise the economic losses associated with the disease.